Title
The effects of the bacteria, Pf-5, and its metabolite, DAPG, on the innate immune response of Drosophila melanogaster.
Date
5-30-2013 2:00 PM
Location
Werner University Center (WUC), Pacific Room
Department
Biology
Session Chair
Ava Howard
Session Chair
Jeffrey Snyder
Session Title
Research in the Biological Sciences
Faculty Sponsor(s)
Kristin Latham
Presentation Type
Poster session
Abstract
The common fruit fly, Drosophila melanogaster, is an ideal organism for studying immune response due to its rapid and well studied life cycle, and that innate immune responses are found in all animals. Upon ingestion of Pseudomonas fluorescens bacteria, Pf-5, in Kings media, or the excreted metabolite 2,4-Diacetylphloroglucinol, DAPG, from the Pf-5 bacteria in Kings media, Drosophila melanogaster larvae exhibit melanization. Melanization is a hyperactive immune response that leads to death. Larvae were staged and fed known concentrations of either Pf-5 bacteria cultured in Kings media, or DAPG in experimental groups. Plates with developing larvae were monitored every other day for larval growth, morphological defects or death. Results showed that different concentrations of Pf-I bacteria, and DAPG metabolites, affected the immune response in Drosophila. This suggests that different concentrations of either bacterial metabolites or the bacteria may be responsible for the immune response in Drosophila.
Recommended Citation
Parker, Ryan, "The effects of the bacteria, Pf-5, and its metabolite, DAPG, on the innate immune response of Drosophila melanogaster." (2013). Academic Excellence Showcase Schedule. 10.
http://digitalcommons.wou.edu/aes_event/2013/biol/10
The effects of the bacteria, Pf-5, and its metabolite, DAPG, on the innate immune response of Drosophila melanogaster.
Werner University Center (WUC), Pacific Room
The common fruit fly, Drosophila melanogaster, is an ideal organism for studying immune response due to its rapid and well studied life cycle, and that innate immune responses are found in all animals. Upon ingestion of Pseudomonas fluorescens bacteria, Pf-5, in Kings media, or the excreted metabolite 2,4-Diacetylphloroglucinol, DAPG, from the Pf-5 bacteria in Kings media, Drosophila melanogaster larvae exhibit melanization. Melanization is a hyperactive immune response that leads to death. Larvae were staged and fed known concentrations of either Pf-5 bacteria cultured in Kings media, or DAPG in experimental groups. Plates with developing larvae were monitored every other day for larval growth, morphological defects or death. Results showed that different concentrations of Pf-I bacteria, and DAPG metabolites, affected the immune response in Drosophila. This suggests that different concentrations of either bacterial metabolites or the bacteria may be responsible for the immune response in Drosophila.