Title

Fruit Flies Eat More Than Fruit: the Toxic Effects of Pseudomonas fluorescens

Date

5-26-2016 4:30 PM

End Time

26-5-2016 4:45 PM

Location

WUC Columbia Room

Department

Biology

Session Chair

Kristin Latham

Session Chair

Jeff Snyder

Session Title

Research in the Biological Sciences

Faculty Sponsor(s)

Kristin Latham and Patricia Flatt

Presentation Type

Presentation

Abstract

Drosophila melanogaster, the fruit fly, is a model organism with genetic applications and an accelerated life cycle making it an ideal subject for developmental and immune system research. There has been little research investigating the lethal and non-lethal responses of flies infected with bacteria early in development. To further the knowledge on the early developmental responses of their immune system, this study examines flies that were exposed to wild type and mutant strains of typically encountered soil bacteria, Pseudomonas fluorescens. It was hypothesized that ingesting the bacteria would cause growth delays coupled with high mortality. Two days post hatching, larvae were fed either strain Pf-5, strain A506, Pf-5 extract or Pf-5 media in one dose. D. melanogaster were then observed throughout the rest of development. Depending on the treatment, it was found that developmental delay and lethality were both observed. In further applications, this research could give insight to the innate immune response exhibited in humans to bacterial infections.

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May 26th, 4:30 PM May 26th, 4:45 PM

Fruit Flies Eat More Than Fruit: the Toxic Effects of Pseudomonas fluorescens

WUC Columbia Room

Drosophila melanogaster, the fruit fly, is a model organism with genetic applications and an accelerated life cycle making it an ideal subject for developmental and immune system research. There has been little research investigating the lethal and non-lethal responses of flies infected with bacteria early in development. To further the knowledge on the early developmental responses of their immune system, this study examines flies that were exposed to wild type and mutant strains of typically encountered soil bacteria, Pseudomonas fluorescens. It was hypothesized that ingesting the bacteria would cause growth delays coupled with high mortality. Two days post hatching, larvae were fed either strain Pf-5, strain A506, Pf-5 extract or Pf-5 media in one dose. D. melanogaster were then observed throughout the rest of development. Depending on the treatment, it was found that developmental delay and lethality were both observed. In further applications, this research could give insight to the innate immune response exhibited in humans to bacterial infections.