Title

Everyone Poops, But What’s in it?

Date

5-26-2016 4:15 PM

End Time

26-5-2016 4:30 PM

Location

WUC Columbia Room

Department

Biology

Session Chair

Kristin Latham

Session Chair

Jeff Snyder

Session Title

Research in the Biological Sciences

Faculty Sponsor(s)

Sarah Boomer

Presentation Type

Presentation

Abstract

A microbiome is the collection of microbes that live in or on a multicellular organism in commensal, mutual, or opportunistic relationships with their host. It represents a diverse ecosystem that is important to host development, health, and metabolism. The gut is where most of the microbiome diversity and density is found. Current research into the human gut microbiome shows that the four major bacterial phyla that are present in the human colon are: Firmicutes (64 percent), Bacteroidetes (23 percent), Proteobacteria (8 percent), and Actinobacteria (3 percent). My research is a preliminary study to develop a cost efficient and accurate method for comparing the gut microbiomes of terrestrial gastropods using a combination of metagenomic analysis and fluorescent microscopy. Samples of feces from the slug Arion sp. were used in a metagenomic analysis to establish a gut microbiome, demonstrating 85 percent Gammaproteobacteria and 15 percent Firmicutes. Fluorescently labeled oligonucleotide probes for Gammaproteobacteria were used to visualize the bacteria present in the feces of the land snail Helix aspersa. The methods from this study will be used in a gut microbiome comparison project with H. aspersa next year.

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May 26th, 4:15 PM May 26th, 4:30 PM

Everyone Poops, But What’s in it?

WUC Columbia Room

A microbiome is the collection of microbes that live in or on a multicellular organism in commensal, mutual, or opportunistic relationships with their host. It represents a diverse ecosystem that is important to host development, health, and metabolism. The gut is where most of the microbiome diversity and density is found. Current research into the human gut microbiome shows that the four major bacterial phyla that are present in the human colon are: Firmicutes (64 percent), Bacteroidetes (23 percent), Proteobacteria (8 percent), and Actinobacteria (3 percent). My research is a preliminary study to develop a cost efficient and accurate method for comparing the gut microbiomes of terrestrial gastropods using a combination of metagenomic analysis and fluorescent microscopy. Samples of feces from the slug Arion sp. were used in a metagenomic analysis to establish a gut microbiome, demonstrating 85 percent Gammaproteobacteria and 15 percent Firmicutes. Fluorescently labeled oligonucleotide probes for Gammaproteobacteria were used to visualize the bacteria present in the feces of the land snail Helix aspersa. The methods from this study will be used in a gut microbiome comparison project with H. aspersa next year.