Title

Effect of Pseudomonas fluorescens on Drosophila larvae

Date

5-28-2015 2:00 PM

End Time

28-5-2015 4:00 PM

Location

Werner University Center (WUC) Pacific Room

Department

Biology

Session Chair

Ava Howard

Session Chair

Jeffrey Snyder

Session Title

Research in the Biological Sciences

Faculty Sponsor(s)

Kristin Latham

Presentation Type

Poster session

Abstract

Drosophila melanogaster, the fruit fly, is a well-established model organism great for studying life cycle development and immune responses for toxins at the cellular level. However, not much is known about the ramifications of introducing harmful bacteria at the first instar larvae stage. We have infected these larvae with different strains of the bacteria Pseudomonas fluorescens to see how this affects the ability to survive and complete their lifecycle. We introduced P. fluorescens (Pf-5, A506 and SWB25) into the food of the first instar larvae to study the toxicity after infection. All strains showed developmental differences. Pf-5 showed a decreased rate of development and survival in a dose dependent fashion when compared to the control. The A506 strain exhibited slight developmental delay and large differences in survival to the adult stage when compared to the control. The SWB25 strain also had decreased survival getting to the adult stage in the wild type bacteria compared to the control. Both A506 and SWB25 Gac- mutants seemed to show similar, if not better, survival rates than the control group. These experiments show that infection of P. fluorescens in D. melanogaster larvae cause developmental delay and lethality before the adult stage.

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May 28th, 2:00 PM May 28th, 4:00 PM

Effect of Pseudomonas fluorescens on Drosophila larvae

Werner University Center (WUC) Pacific Room

Drosophila melanogaster, the fruit fly, is a well-established model organism great for studying life cycle development and immune responses for toxins at the cellular level. However, not much is known about the ramifications of introducing harmful bacteria at the first instar larvae stage. We have infected these larvae with different strains of the bacteria Pseudomonas fluorescens to see how this affects the ability to survive and complete their lifecycle. We introduced P. fluorescens (Pf-5, A506 and SWB25) into the food of the first instar larvae to study the toxicity after infection. All strains showed developmental differences. Pf-5 showed a decreased rate of development and survival in a dose dependent fashion when compared to the control. The A506 strain exhibited slight developmental delay and large differences in survival to the adult stage when compared to the control. The SWB25 strain also had decreased survival getting to the adult stage in the wild type bacteria compared to the control. Both A506 and SWB25 Gac- mutants seemed to show similar, if not better, survival rates than the control group. These experiments show that infection of P. fluorescens in D. melanogaster larvae cause developmental delay and lethality before the adult stage.