Title

Pseudomonas fluorescens ingestion by Drosophila melanogaster larvae

Date

5-28-2015 2:00 PM

End Time

28-5-2015 4:00 PM

Location

Werner University Center (WUC) Pacific Room

Department

Biology

Session Chair

Ava Howard

Session Chair

Jeffrey Snyder

Session Title

Research in the Biological Sciences

Faculty Sponsor(s)

Kristin Latham

Presentation Type

Poster session

Abstract

The fruit fly, Drosophila melanogaster, has been used as a model organism to study host-microbe interactions and host immune response. After ingestion of Pseudomonas fluorescens bacteria, Drosophila melanogaster show morphological defects, delayed development or death. I experimented with different strains of P. fluorescens Pf-5, A506 and SWB25 bacteria and their respective Gac mutants to determine how infection alters the ability of larvae to survive and complete development. Larvae were staged and fed known doses of P. fluorescens strains. The developing larvae plates were monitored daily for larval growth, morphological defects or death, as compared to controls. Larvae treated with P. fluorescens Pf-5 showed delayed development. Interestingly, 61.1% of larvae reached the adult stage when treated with Pf-5 compared to 79.41% of control larvae. Surprisingly, 42.86% of larvae treated with A506 reached the adult stage compared to 78.95 % of control and 85.45 % of Gac mutants. 80.39% treated with SWB25 reached the adult stage compared to 93.93 % of control and 85.74 % of Gac. The experiments demonstrate that ingestion of Pseudomonas by Drosophila causes lethal phenotypes including delayed development and metamorphosis. The results also suggest that bacterial metabolites are responsible for the toxicity response in Drosophila because Gac mutants showed greater survival compared to wild type.

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May 28th, 2:00 PM May 28th, 4:00 PM

Pseudomonas fluorescens ingestion by Drosophila melanogaster larvae

Werner University Center (WUC) Pacific Room

The fruit fly, Drosophila melanogaster, has been used as a model organism to study host-microbe interactions and host immune response. After ingestion of Pseudomonas fluorescens bacteria, Drosophila melanogaster show morphological defects, delayed development or death. I experimented with different strains of P. fluorescens Pf-5, A506 and SWB25 bacteria and their respective Gac mutants to determine how infection alters the ability of larvae to survive and complete development. Larvae were staged and fed known doses of P. fluorescens strains. The developing larvae plates were monitored daily for larval growth, morphological defects or death, as compared to controls. Larvae treated with P. fluorescens Pf-5 showed delayed development. Interestingly, 61.1% of larvae reached the adult stage when treated with Pf-5 compared to 79.41% of control larvae. Surprisingly, 42.86% of larvae treated with A506 reached the adult stage compared to 78.95 % of control and 85.45 % of Gac mutants. 80.39% treated with SWB25 reached the adult stage compared to 93.93 % of control and 85.74 % of Gac. The experiments demonstrate that ingestion of Pseudomonas by Drosophila causes lethal phenotypes including delayed development and metamorphosis. The results also suggest that bacterial metabolites are responsible for the toxicity response in Drosophila because Gac mutants showed greater survival compared to wild type.