Title

Wood smoke particles generate free radicals and cause lipid peroxidation, DNA damage, NFkB activation and TNF-a release in macrophages

Date

5-29-2014 9:00 AM

End Time

29-5-2014 11:00 AM

Location

Werner University Center (WUC) Pacific Room

Department

Chemistry

Session Chair

Patricia Flatt

Session Title

Biochemistry Posters

Faculty Sponsor(s)

Patricia Flatt

Presentation Type

Poster session

Abstract

This study investigated the generation of free radicals created by wood smoke and the cellular damage that was caused. Electron spin resonance techniques measured reactive oxygen species in wood smoke and found that hydroxyl radicals were generated. DNA strand breakage was also seen by exposing wood smoke to l Hind III fragments using gel electrophoresis. Cellular DNA damage was also observed in cultured mouse macrophage cells within the single cell gel electrophoresis assay. These results indicate that the free radicals generated by wood smoke through the reaction of Fe with H2O2 are able to cause DNA and cellular damage.

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May 29th, 9:00 AM May 29th, 11:00 AM

Wood smoke particles generate free radicals and cause lipid peroxidation, DNA damage, NFkB activation and TNF-a release in macrophages

Werner University Center (WUC) Pacific Room

This study investigated the generation of free radicals created by wood smoke and the cellular damage that was caused. Electron spin resonance techniques measured reactive oxygen species in wood smoke and found that hydroxyl radicals were generated. DNA strand breakage was also seen by exposing wood smoke to l Hind III fragments using gel electrophoresis. Cellular DNA damage was also observed in cultured mouse macrophage cells within the single cell gel electrophoresis assay. These results indicate that the free radicals generated by wood smoke through the reaction of Fe with H2O2 are able to cause DNA and cellular damage.