Title

Drosophila melanogaster development after ingestion of Pseudomonas fluorescens bacterial strains

Date

5-29-2014 2:00 PM

End Time

29-5-2014 4:00 PM

Location

Werner University Center (WUC) Pacific Room

Department

Biology

Session Chair

Ava Howard

Session Title

Research in the Biological Sciences

Faculty Sponsor(s)

Kristin Latham

Presentation Type

Poster session

Abstract

The fruit fly, Drosophila melanogaster, has been used as a model organism to study host-microbe interactions and host immune response. After ingestion of Pseudomonas fluorescens bacteria, Drosophila melanogaster show morphological defects, delayed development or death. In our lab, we experimented with different strains of Pseudomonas fluorescens Pf-5, A506 and SWB25 bacteria to determine how ingestion alters the ability of larvae to survive and complete development. Larvae were staged and fed known doses of P. fluorescens strains. The developing larvae plates were monitored every other day for larval growth, morphological defects or death, as compared to controls. Larvae treated with P. fluorescens Pf-5 showed delayed development. Interestingly, 32% of larvae reached the adult stage when treated with Pf-5 compared to 80% of control larvae. Surprisingly, 93% of larvae treated with A506 reached the adult stage and 96% treated with SWB25 reached adult stage. However, neither strain showed morphological defects. The experiments demonstrate that ingestion of Pseudomonas by Drosophila can be lethal and delay Drosophila development. The results also suggest that bacterial metabolites are responsible for the toxicity response in Drosophila.

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May 29th, 2:00 PM May 29th, 4:00 PM

Drosophila melanogaster development after ingestion of Pseudomonas fluorescens bacterial strains

Werner University Center (WUC) Pacific Room

The fruit fly, Drosophila melanogaster, has been used as a model organism to study host-microbe interactions and host immune response. After ingestion of Pseudomonas fluorescens bacteria, Drosophila melanogaster show morphological defects, delayed development or death. In our lab, we experimented with different strains of Pseudomonas fluorescens Pf-5, A506 and SWB25 bacteria to determine how ingestion alters the ability of larvae to survive and complete development. Larvae were staged and fed known doses of P. fluorescens strains. The developing larvae plates were monitored every other day for larval growth, morphological defects or death, as compared to controls. Larvae treated with P. fluorescens Pf-5 showed delayed development. Interestingly, 32% of larvae reached the adult stage when treated with Pf-5 compared to 80% of control larvae. Surprisingly, 93% of larvae treated with A506 reached the adult stage and 96% treated with SWB25 reached adult stage. However, neither strain showed morphological defects. The experiments demonstrate that ingestion of Pseudomonas by Drosophila can be lethal and delay Drosophila development. The results also suggest that bacterial metabolites are responsible for the toxicity response in Drosophila.