Title

Effects of Bacterial Metabolites on Fruit Fly Development

Date

5-29-2014 2:00 PM

End Time

29-5-2014 4:00 PM

Location

Werner University Center (WUC) Pacific Room

Department

Biology

Session Chair

Ava Howard

Session Title

Research in the Biological Sciences

Faculty Sponsor(s)

Kristin Latham

Presentation Type

Poster session

Abstract

Bacterial metabolites are known to affect the development of fruit flies (Drosophila melanogastor). Pseudomonas fluorescens (Pf-5), a common gram negative bacterium, produces a variety of metabolites such as pyoluteorin, pyrrolnitrin, rhizoxin, and cyanide. Different strains of Pf-5 can be created via DNA insertions or deletions. This can alter metabolite production of the bacteria. Our goal is to determine the effects of several Pf-5 strains on fruit fly development. Each experimental round consisted of one strain of Pf-5 and its associated Gac mutant. Plates were set up for a Pf-5 strain, its associated Gac mutant, and controls. Each plate started with the same number of fly eggs each round and development stages were checked every two days for two weeks. Survival in the A-506 strain round was lowest for adults. Survival in the SWB25 strain round was similar among all plates through all life stages. Our findings suggest that the A-506 non-gac mutant strain has the greatest impact on proper development of fruit flies.

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May 29th, 2:00 PM May 29th, 4:00 PM

Effects of Bacterial Metabolites on Fruit Fly Development

Werner University Center (WUC) Pacific Room

Bacterial metabolites are known to affect the development of fruit flies (Drosophila melanogastor). Pseudomonas fluorescens (Pf-5), a common gram negative bacterium, produces a variety of metabolites such as pyoluteorin, pyrrolnitrin, rhizoxin, and cyanide. Different strains of Pf-5 can be created via DNA insertions or deletions. This can alter metabolite production of the bacteria. Our goal is to determine the effects of several Pf-5 strains on fruit fly development. Each experimental round consisted of one strain of Pf-5 and its associated Gac mutant. Plates were set up for a Pf-5 strain, its associated Gac mutant, and controls. Each plate started with the same number of fly eggs each round and development stages were checked every two days for two weeks. Survival in the A-506 strain round was lowest for adults. Survival in the SWB25 strain round was similar among all plates through all life stages. Our findings suggest that the A-506 non-gac mutant strain has the greatest impact on proper development of fruit flies.