Title

The effects of the bacteria, Pf-5, and its metabolite, DAPG, on the innate immune response of Drosophila melanogaster.

Date

5-30-2013 2:00 PM

Location

Werner University Center (WUC), Pacific Room

Department

Biology

Session Chair

Ava Howard

Session Chair

Jeffrey Snyder

Session Title

Research in the Biological Sciences

Faculty Sponsor(s)

Kristin Latham

Presentation Type

Poster session

Abstract

The common fruit fly, Drosophila melanogaster, is an ideal organism for studying immune response due to its rapid and well studied life cycle, and that innate immune responses are found in all animals. Upon ingestion of Pseudomonas fluorescens bacteria, Pf-5, in Kings media, or the excreted metabolite 2,4-Diacetylphloroglucinol, DAPG, from the Pf-5 bacteria in Kings media, Drosophila melanogaster larvae exhibit melanization. Melanization is a hyperactive immune response that leads to death. Larvae were staged and fed known concentrations of either Pf-5 bacteria cultured in Kings media, or DAPG in experimental groups. Plates with developing larvae were monitored every other day for larval growth, morphological defects or death. Results showed that different concentrations of Pf-I bacteria, and DAPG metabolites, affected the immune response in Drosophila. This suggests that different concentrations of either bacterial metabolites or the bacteria may be responsible for the immune response in Drosophila.

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May 30th, 2:00 PM

The effects of the bacteria, Pf-5, and its metabolite, DAPG, on the innate immune response of Drosophila melanogaster.

Werner University Center (WUC), Pacific Room

The common fruit fly, Drosophila melanogaster, is an ideal organism for studying immune response due to its rapid and well studied life cycle, and that innate immune responses are found in all animals. Upon ingestion of Pseudomonas fluorescens bacteria, Pf-5, in Kings media, or the excreted metabolite 2,4-Diacetylphloroglucinol, DAPG, from the Pf-5 bacteria in Kings media, Drosophila melanogaster larvae exhibit melanization. Melanization is a hyperactive immune response that leads to death. Larvae were staged and fed known concentrations of either Pf-5 bacteria cultured in Kings media, or DAPG in experimental groups. Plates with developing larvae were monitored every other day for larval growth, morphological defects or death. Results showed that different concentrations of Pf-I bacteria, and DAPG metabolites, affected the immune response in Drosophila. This suggests that different concentrations of either bacterial metabolites or the bacteria may be responsible for the immune response in Drosophila.